You can estimate the molecular weight of your protein of interest by simply comparing the position of its’ band on the gel with those of the protein standards. You can find some helpful tips on western blotting here.Ģ. Run your western blot with your protein of interest and the molecular weight standards. If you want to do this, you will need to run your sample on the same gel as a set of molecular weight standards (proteins of known molecular weight.)ġ. You can use the R f value to calculate the molecular weight of a protein of interest. Migration distance of the dye front So why do we need the Rf value? In a previous post, we looked at how to calculate the R f value of a protein from a western blot. Using R f value to calculate molecular weight
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